polyclonal anti-src [py418] phospho-specific antibody  (Thermo Fisher)

Journal: Cancer cell

Article Title: Aberrant ERBB4-SRC Signaling as a Hallmark of Group 4 Medulloblastoma Revealed by Integrative Phosphoproteomic Profiling.

doi: 10.1016/j.ccell.2018.08.002

Figure Lengend Snippet: Figure 6. Aberrant Receptor Tyrosine Kinase Signaling Pathway in G4 MBs (A) Western blot analysis of global tyrosine phosphorylation in primary G3 and G4 MBs using an anti-phospho-tyrosine antibody. (B) Analysis of ERBB phosphorylation in primary MBs using phospho-receptor tyrosine kinase arrays. Global arrays are shown in the upper part or the panel. A close-up of the ERBB family of receptor tyrosine kinases is shown in the bottom panel. (C) Western blot analysis of the indicated proteins and specific phosphorylation sites in primary MBs corresponding to samples used in Figure 6A. The actin blot shown in Figure 6C was used as a loading control for the blots in Figures 6A and 6C. (D and E) Correlation plot between mRNA levels (y axis) and DNA methylation status (x axis) for GRB2 (Cavalli et al., 2017; llmnhm450 [cg24244854] versus Hugene11t [8018364]) (D) and ARRB1 (Cavalli et al., 2017; llmnhm450 [cg02449461] versus Hugene11t [7950473]) (E) according to the indicated MB subgroups. (F and G) Correlation plot between mRNA levels (y axis) and DNA methylation status (x axis) for GRB2 (Cavalli et al., 2017; GRB2, llmnhm450 [cg11818853] versus Hugene11t [8018364]) (F) and ARRB1 (Cavalli et al., 2017; llmnhm450 [cg11818853] versus Hugene11t [8018364]) (G) according to the indicated chromosomal copy-number alterations. Boxplots show median (line), upper and lower quartiles (boxes), and lines extending to highest and lowest observations (whiskers). (H) Immunohistochemistry of sagittal sections of mouse developing cerebellum at embryonic day 13.5 (E13.5) and postnatal day 7 (P7) for ERBB4, pY418-SRC, and p-Tyr. Scale bars are indicated. See also Figure S7.

Article Snippet: For protein detection, we used the following primary antibodies: P-Tyr (Cell signaling #8954), ERBB4 (Invitrogen #MA1-861), pY418-SRC (R&D systems #AF2685), SRC (Cell signaling #2109), P-AKT (Cell signaling #9271), P-ERK (Cell signaling #9106), and Actin (Santa Cruz #SC-1615).

Techniques: Western Blot, Phospho-proteomics, Control, DNA Methylation Assay, Immunohistochemistry

Journal: Inflammation

Article Title: Focal Adhesion Kinase Activity and Localization is Critical for TNF-α-Induced Nuclear Factor-κB Activation

doi: 10.1007/s10753-020-01408-5

Figure Lengend Snippet: Antibodies Used in This Study

Article Snippet: pY418 Src , Cell Signal Technology, 2101 , Rabbit , 1:2000 (WB) , AB_331697.

Techniques: Recombinant